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Ultrasound as a safe and reliable guidance for subarachnoid puncture in rabbits
Pereira, Ivan Dias Fernandes; Vital, Roberto Bezerra; Silva, Ronaldo; Valerini, Felipe Gilberto; Machado, Vania Maria de Vasconcelos; Marques, Mariangela Esrher; Miot, Helio; Navarro, Lais Helena; Ganem, Eliana Marisa.
  • Pereira, Ivan Dias Fernandes; Universidade Estadual Paulista. Department of Anesthesiology. Botucatu. BR
  • Vital, Roberto Bezerra; Universidade Estadual Paulista. Department of Anesthesiology. Botucatu. BR
  • Silva, Ronaldo; Universidade Estadual Paulista. Department of Anesthesiology. Botucatu. BR
  • Valerini, Felipe Gilberto; Universidade Estadual Paulista. Department of Anesthesiology. Botucatu. BR
  • Machado, Vania Maria de Vasconcelos; Universidade Estadual Paulista. Department of Anesthesiology. Botucatu. BR
  • Marques, Mariangela Esrher; Universidade Estadual Paulista. Department of Anesthesiology. Botucatu. BR
  • Miot, Helio; Universidade Estadual Paulista. Department of Anesthesiology. Botucatu. BR
  • Navarro, Lais Helena; Universidade Estadual Paulista. Department of Anesthesiology. Botucatu. BR
  • Ganem, Eliana Marisa; Universidade Estadual Paulista. Department of Anesthesiology. Botucatu. BR
Acta cir. bras ; 32(1): 14-21, Jan. 2017. tab, graf
Article Dans Anglais | LILACS | ID: biblio-837670
ABSTRACT
Abstract

Purpose:

To evaluate a model for studying the toxicity in nervous tissue and meninges using ultrasound to guide needle insertion into the subarachnoid space of rabbits, with the objective of avoiding injuries triggered by the puncture and by intraneural injection of solutions.

Methods:

Forty-five adult female rabbits were divided into 3 groups (G) G1 underwent subarachnoid puncture, G2 underwent subarachnoid injection of saline solution and G3 underwent subarachnoid injection of 0.5% hyperbaric bupivacaine. A needle was inserted into the S1-S2 subarachnoid space guided by ultrasound. The sensitivity and motility of the animals were evaluated for 3 days, after which the animals were sacrificed for removal of lumbar and sacral portions of the spinal cord for histological examination by light microscopy and immunohistochemistry.

Results:

All animals had motor function and pain sensitivity on the evaluation period. No animal had complication during or after the procedures.

Conclusion:

Ultrasound is a useful and safe method for the correct identification of the subarachnoid space of rabbits.
Sujets)


Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Ponction lombaire / Espace sous-arachnoïdien / Échographie interventionnelle Type d'étude: Guide de pratique / Étude pronostique Limites du sujet: Animaux langue: Anglais Texte intégral: Acta cir. bras Thème du journal: Chirurgie générale / Procedimentos Cir£rgicos Operat¢rios Année: 2017 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Universidade Estadual Paulista/BR

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Ponction lombaire / Espace sous-arachnoïdien / Échographie interventionnelle Type d'étude: Guide de pratique / Étude pronostique Limites du sujet: Animaux langue: Anglais Texte intégral: Acta cir. bras Thème du journal: Chirurgie générale / Procedimentos Cir£rgicos Operat¢rios Année: 2017 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Universidade Estadual Paulista/BR