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Protein carbonyl content: a novel biomarker for aging in HIV/AIDS patients
Kolgiri, Vaishali; Patil, Vinayak Wamanrao.
  • Kolgiri, Vaishali; Grant Government Medical College and Sir JJ Group of Hospitals. Department of Biochemistry. Mumbai. IN
  • Patil, Vinayak Wamanrao; Grant Government Medical College and Sir JJ Group of Hospitals. Department of Biochemistry. Mumbai. IN
Braz. j. infect. dis ; 21(1): 35-41, Jan.-Feb. 2017. tab, graf
Article Dans Anglais | LILACS | ID: biblio-839181
ABSTRACT
Abstract

Background:

The major complications of “treated” Human Immunodeficiency Virus (HIV) infection are cardiovascular disease, malignancy, renal disease, liver disease, bone disease, and perhaps neurological complications, which are phenomena of the normal aging process occurring at an earlier age in the HIV-infected population. The present study is aimed to explore protein carbonyl content as a biomarker for detecting oxidative DNA damage induced ART toxicity and/or accelerated aging in HIV/AIDS patients.

Objective:

To investigate the potential of carbonyl content as a biomarker for detecting oxidative Deoxyribonucleic acid (DNA) damage induced Antiretroviral Theraphy (ART) toxicity and/or accelerated aging in HIV/AIDS patients.

Methods:

In this case–control study a total 600 subjects were included. All subjects were randomly selected and grouped as HIV-negative (control group) (n = 300), HIV-infected ART naive (n = 100), HIV-infected on first line ART (n = 100), and HIV-infected on second line ART (n = 100). Seronegative control subjects were age- and sex-matched with the ART naive patients and the two other groups. Carbonyl protein was determined by the method described in Levine et al. DNA damage marker 8-OH-dG was determined using 8-hydroxy-2-deoxy Guanosine StressXpress ELA Kit by StressMarq Biosciences.

Results:

Protein carbonyl content levels and oxidative DNA damage were significantly higher (p < 0.05) in HIV-infected patients on second line ART and HIV-infected patients on first line ART than ART naive patients and controls. In a linear regression analysis, increased protein carbonyl content was positively associated with increased DNA damage (OR 0.356; 95% CI 0.287–0.426) p < 0.05.

Conclusions:

Carbonyl content may has a role as a biomarker for detecting oxidative DNA damage induced ART toxicity and/or accelerated aging in HIV/AIDS patients. Larger studies are warranted to elucidate the role of carbonyl content as a biomarker for premature aging in HIV/AIDS patients.
Sujets)


Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Altération de l&apos;ADN / Vieillissement / Syndrome d&apos;immunodéficience acquise féline / Syndrome d&apos;immunodéficience acquise / Thérapie antirétrovirale hautement active / Désoxyguanosine / Carbonylation des protéines Type d'étude: Étude observationnelle / Facteurs de risque Limites du sujet: Animaux / Femelle / Humains / Mâle langue: Anglais Texte intégral: Braz. j. infect. dis Thème du journal: Maladies transmissibles Année: 2017 Type: Article Pays d'affiliation: Inde Institution/Pays d'affiliation: Grant Government Medical College and Sir JJ Group of Hospitals/IN

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Altération de l&apos;ADN / Vieillissement / Syndrome d&apos;immunodéficience acquise féline / Syndrome d&apos;immunodéficience acquise / Thérapie antirétrovirale hautement active / Désoxyguanosine / Carbonylation des protéines Type d'étude: Étude observationnelle / Facteurs de risque Limites du sujet: Animaux / Femelle / Humains / Mâle langue: Anglais Texte intégral: Braz. j. infect. dis Thème du journal: Maladies transmissibles Année: 2017 Type: Article Pays d'affiliation: Inde Institution/Pays d'affiliation: Grant Government Medical College and Sir JJ Group of Hospitals/IN