An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules
Braz. J. Pharm. Sci. (Online)
;
53(2): e16064, 2017. tab, graf
Article
Dans Anglais
| LILACS
| ID: biblio-839484
ABSTRACT
ABSTRACT Prednisone is an anti-inflammatory steroid drug widely used in clinical practice. However, no high-performance liquid chromatographic (HPLC) method has been described in the literature for the determination of prednisone in capsules until now. Thus, an HPLC method was developed using a C18 (250x4.0, 5 µm) column, with methanolwater (7030) as mobile phase at a flow rate of 1 mL/min and detection at 240 nm. The developed method was validated following current Brazilian legislation. Additionally, linearity was assessed by evaluating the assumptions of normality, homoscedasticity, and independency of residuals, and the fit to the linear model. The method showed linearity (r2>0.99) over the range of 14.0-26.0 µg/mL, selectivity, precision (RSD<2.0%), robustness, and accuracy (average recovery of 100.05%). The chromatographic procedure was applied for assay and uniformity content determination of three different batches of prednisone capsules, showing to be suitable for their quality control.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Capsules
/
Prednisone
/
Chromatographie en phase liquide à haute performance
langue:
Anglais
Texte intégral:
Braz. J. Pharm. Sci. (Online)
Thème du journal:
Farmacologia
/
Teraputica
/
Toxicologia
Année:
2017
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Federal University of Minas Gerais/BR
/
Oswaldo Cruz Foundation/BR
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