Presença do genoma de BoHV-5 no líquido cefalorraquidiano de bovinos com meningoencefalite herpética / Presence of BoHV-5 genome in cerebrospinal fluid of cattle with herpetic meningoencephalitis
Arq. bras. med. vet. zootec. (Online)
;
68(2): 548-552, mar.-abr. 2016. ilus, tab
Article
Dans Portugais
| LILACS, VETINDEX
| ID: biblio-874957
ABSTRACT
The diagnosis of bovine herpesvirus 5 (BoHV-5) encephalitis is confirmed after death by laboratory methods applied to brain fragments. Alternative methods to confirm ante-mortem diagnosis are important because the disease is not always lethal. The aim of this study was to investigate whether the presence of the virus genome in the cerebrospinal fluid (CSF) detected by polymerase chain reaction (PCR) might be admitted as a method for ante-mortem diagnosis. CSF samples were taken from 14 animals suffering from BoHV-5 encephalitis, diagnosed by characteristic histopathological lesions in the brain and by identification of the virus genome by PCR in different portions of the brain. Virus DNA was detected in the CSF of 21.42% (3/14) of the evaluated animals. Ante-mortem detection of the virus genome in the CSF showed low sensitivity to confirm the diagnosis. The diagnosis is confirmed by a positive result but a negative one does not discard the disease.(AU)
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Liquide cérébrospinal
/
Réaction de polymérisation en chaîne
/
Herpèsvirus bovin de type 5
/
Méningoencéphalite
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Portugais
Texte intégral:
Arq. bras. med. vet. zootec. (Online)
Année:
2016
Type:
Article
/
descriptif de projet
Institution/Pays d'affiliation:
Universidade Estadual de Londrina/BR
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