Efficacy of phosphocreatine pre-administration on XIAP and Smac in ischemic penumbra of rats with focal cerebral ischemia reperfusion injury
Acta cir. bras
;
33(2): 117-124, Feb. 2018. tab, graf
Article
Dans Anglais
| LILACS
| ID: biblio-886260
ABSTRACT
Abstract Purpose:
To observe the efficacy of phosphocreatine pre-administration (PCr-PA) on X-linked inhibitor of apoptosis protein (XIAP), the second mitochondia-derived activator of caspase (Smac) and apoptosis in the ischemic penumbra of rats with focal cerebral ischemia-reperfusion injury (CIRI).Methods:
A total of 60 healthy male Sprague Dawley (SD) rats were randomly divided into three groups (n=20) group A (the sham operation group), group B <intraperitoneally injected with 20 mg/kg (10 mg/ml) of saline before preparing the ischemia-reperfusion (IR) model>, and group C <intraperitoneally injected with 20 mg/kg (10 mg/ml) of PCr immediately before preparing the IR model>. After 24 h for reperfusion, the neurological function was evaluated and the tissue was sampled to detect expression of XIAP, Smac and caspase-3 positive cells in the ischemic penumbra so as to observe the apoptosis.Results:
Compared with group B, neurological deficit scores, numbers of apoptotic cells, expression of Smac,caspase-9 and the numbers of Caspase-3 positive cells were decreased while expression of XIAP were increased in the ischemic penumbra of group C.Conclusions:
Phosphocreatine pre-administration may elicit neuroprotective effects in the brain by increasing expression of X-linked inhibitor of apoptosis protein, reducing expression of second mitochondia-derived activator of caspase, and inhibiting the apoptosis in the ischemic penumbra.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Phosphocréatine
/
Cardiotoniques
/
Lésion d'ischémie-reperfusion
/
Encéphalopathie ischémique
/
Protéines mitochondriales
/
Protéines et peptides de signalisation intracellulaire
/
Protéine inhibitrice de l'apoptose liée au chromosome X
Type d'étude:
Essai clinique contrôlé
/
Étude pronostique
Limites du sujet:
Animaux
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Acta cir. bras
Thème du journal:
Chirurgie générale
/
Procedimentos Cir£rgicos Operat¢rios
Année:
2018
Type:
Article
Pays d'affiliation:
Chine
Institution/Pays d'affiliation:
Nanjing Medical University/CN
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