Characterization and bioactivities of a novel polysaccharide obtained from Gracilariopsis lemaneiformis
An. acad. bras. ciênc
;
89(1): 175-189, Jan,-Mar. 2017. graf
Article
Dans Anglais
| LILACS
| ID: biblio-886635
ABSTRACT
ABSTRACT Gracilariopsis lemaneiformis is a type of red alga that contains seaweed polysaccharide agar. In this study, a novel non-agar seaweed polysaccharide fraction named GCP (short of crude polysaccharide obtained from Gracilariopsis lemaneiformis) was isolated from Gracilariopsis lemaneiformis. Structural analysis showed that GCP shows triple helical chain conformation when dissolved in water and has many branches and long side chains. Also, 1→3 linkage is the major linkage and the sugar structures are galactopyranose configurations linked by β-type glycosidic linkages. Two macromolecular substance fractions (GCP-1 and GCP-2) were purified by DEAE Sepharose Fast Flow column chromatography. Moreover, a splenocyte damage assay and splenocyte proliferation assay were used to analyse the bioactivities of GCP, GCP-1 and GCP-2. It was demonstrated that polysaccharides could protect splenocyte damaged by H2O2; GCP-2 shows a greatest protection rate, that is, 92.8%, which significantly enhanced the splenocyte proliferation, and GCP showed the highest proliferation rate, 9.30%. The results suggested that this type of novel non-agar polysaccharide displayed remarkable antioxidant and immunomodulatory activities and early alkali treatment could decrease the activities. It may represent a potential material for health food and clinical medicines.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Polyosides
/
Algue marine
/
Rhodophyta
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
An. acad. bras. ciênc
Thème du journal:
Science
Année:
2017
Type:
Article
Pays d'affiliation:
Chine
Institution/Pays d'affiliation:
Agricultural University of Hebei/CN
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS