Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts
An. acad. bras. ciênc
;
89(3,supl): 2053-2073, 2017. tab, graf
Article
Dans Anglais
| LILACS
| ID: biblio-886784
ABSTRACT
ABSTRACT This study aimed to further investigate the cytotoxicity against tumor cell lines and several bacterial strains of Annona squamosa and its mode of action. Methanol extracts of A. squamosa leaves (ASL) and seeds (ASS) were used. ASL showed significant antibacterial activity against S. aureus, K. pneumoniae and E. faecalis with MIC values of 78, 78 and 39 µg/mL respectively. Moreover, ASL exhibited significant biofilm disruption, rapid time dependent kinetics of bacterial killing, increased membrane permeability and significantly reduced the cell numbers and viability. Regarding the cytotoxicity against tumor cell lines, ASS was more active against Jurkat and MCF-7 cells, with CI50 1.1 and 2.1 µg/mL, respectively. ASL showed promising activity against Jurkat and HL60, with CI50 4.2 and 6.4 µg/mL, respectively. Both extracts showed lower activity against VERO cells and reduced the clonogenic survival at higher concentrations (IC90) to MCF-7 and HCT-116 lineages. The alkaloids anonaine, asimilobine, corypalmine, liriodenine nornuciferine and reticuline were identified in extracts by UPLC-ESI-MS/MS analysis. This study reinforced that A. squamosa presents a remarkable phytomedicinal potential and revealed that its antimicrobial mechanism of action is related to bacterial membrane destabilization.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Staphylococcus aureus
/
Extraits de plantes
/
Enterococcus faecalis
/
Annona
/
Klebsiella pneumoniae
/
Antibactériens
Limites du sujet:
Animaux
/
Humains
langue:
Anglais
Texte intégral:
An. acad. bras. ciênc
Thème du journal:
Science
Année:
2017
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Federal de Juiz de Fora/BR
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