The regulatory role of IL-6R in hepatitis B-associated fibrosis and cirrhosis
Braz. j. med. biol. res
; 50(11): e6246, 2017. graf
Article
Dans En
| LILACS
| ID: biblio-888947
Responsable en Bibliothèque :
BR1.1
ABSTRACT
This study investigated the expression and regulation of IL-6R in hepatitis B-associated moderate hepatic fibrosis and cirrhosis. Liver tissues, peripheral blood monocytes (PBMs) and serum were collected from 26 hepatitis B patients with liver fibrosis and 35 hepatitis B patients with liver cirrhosis. The levels of Il-6r mRNA expression in these samples were examined by quantitative real-time PCR and IL-6R protein levels were analyzed by western blot and ELISA. MiRNAs that regulate IL-6R expression were predicted by bioinformatics analysis, and validated by dual luciferase reporter assay. Compared with the hepatic fibrosis group, IL-6R was significantly upregulated at both mRNA and protein levels in liver tissues, PBMs and serum samples from the hepatic cirrhosis group (P<0.05). The 3′UTR of Il-6r mRNA was predicted to contain a miR-30b binding site and IL-6R was identified as a possible target of miR-30b. MiR-30b expression was significantly downregulated in samples from hepatic cirrhosis patients compared with hepatic fibrosis patients (P<0.05). In conclusion, IL-6R was upregulated while miR-30b was decreased in patients with liver cirrhosis. The miR-30 can directly regulate the expression of IL-6R.
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Récepteurs à l'interleukine-6
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MicroARN
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Hépatite B
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Cirrhose du foie
Type d'étude:
Prognostic_studies
/
Risk_factors_studies
Limites du sujet:
Adult
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Aged
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Female
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Humans
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Male
langue:
En
Texte intégral:
Braz. j. med. biol. res
Thème du journal:
BIOLOGIA
/
MEDICINA
Année:
2017
Type:
Article