Culture of human dental pulp cells at variable times post-tooth extraction
Braz. oral res. (Online)
;
32: e003, 2018. graf
Article
Dans Anglais
| LILACS
| ID: biblio-889476
ABSTRACT
Abstract The aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was tested immediately after extraction; the other groups were each tested at one of the following time points 30 minutes, 1 hour, 2 hours, and 5 hours post-extraction. Cell morphology was analysed by light microscopy; cell proliferation was analysed using MTT assay and by counting the viable cells in a haemocytometer. Similar results were observed in all groups (p < 0.05). A delay of up to five hours for tooth processing and tissue collection does not preclude the establishment of dental pulp cell cultures, affect the morphology of these cells, or reduce their proliferative potential.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Extraction dentaire
/
Techniques de culture cellulaire
/
Pulpe dentaire
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Braz. oral res. (Online)
Thème du journal:
Dentisterie
Année:
2018
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade de Brasília/BR
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