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Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
Paiva, Marcelo Henrique Santos; Guedes, Duschinka Ribeiro Duarte; Leal, Walter Soares; Ayres, Constância Flávia Junqueira.
  • Paiva, Marcelo Henrique Santos; Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru. BR
  • Guedes, Duschinka Ribeiro Duarte; Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru. BR
  • Leal, Walter Soares; Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru. BR
  • Ayres, Constância Flávia Junqueira; Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Caruaru. BR
Genet. mol. biol ; 40(3): 597-599, July-Sept. 2017. tab, graf
Article Dans Anglais | LILACS | ID: biblio-892428
ABSTRACT
Abstract Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).


Texte intégral: Disponible Indice: LILAS (Amériques) Type d'étude: Etude diagnostique langue: Anglais Texte intégral: Genet. mol. biol Thème du journal: Génétique Année: 2017 Type: Article / descriptif de projet Pays d'affiliation: Brésil Institution/Pays d'affiliation: Universidade Federal de Pernambuco/BR

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Texte intégral: Disponible Indice: LILAS (Amériques) Type d'étude: Etude diagnostique langue: Anglais Texte intégral: Genet. mol. biol Thème du journal: Génétique Année: 2017 Type: Article / descriptif de projet Pays d'affiliation: Brésil Institution/Pays d'affiliation: Universidade Federal de Pernambuco/BR