Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells
Rev. Soc. Bras. Med. Trop
;
51(2): 168-173, Mar.-Apr. 2018. graf
Article
Dans Anglais
| LILACS
| ID: biblio-897058
ABSTRACT
Abstract INTRODUCTION:
Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year.METHODS:
In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerase chain reaction (RT-PCR).RESULTS:
All three assays identified 29.1% (39/134) of the patients as dengue-positive. The FACS approach and real-time RT-PCR detected the DENV in 39 and 44 samples, respectively. On the other hand, the immunochromatographic assay detected the NS1 protein in 40.1% (56/134) of the patients. The Cohen's kappa coefficient analysis revealed a substantial agreement among the three methods.CONCLUSIONS:
The FACS approach may be a useful alternative for dengue diagnosis and can be implemented in public and private laboratories.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Agranulocytes
/
Dengue
/
Virus de la dengue
/
Anticorps antiviraux
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Rev. Soc. Bras. Med. Trop
Thème du journal:
Médecine tropicale
Année:
2018
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade de São Paulo/BR
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