IL32 expression in peripheral blood CD3+ cells from myelodysplastic syndromes patients
Appl. cancer res
;
37: 1-6, 2017. tab, ilus
Article
Dans Anglais
| LILACS, Inca
| ID: biblio-915122
ABSTRACT
Background:
Myelodysplastic syndromes (MDS) are a heterogeneous group of disorders characterized by ineffective hematopoiesis and risk of leukemia transformation. There is evidence to suggest the participation of immune system deregulation in MDS pathogenesis. Interleukin-32 (IL-32) is a newly described multifunctional cytokine reported as an important mediator in autoimmune and inflammatory disorders. In the present study, we reported the expression of IL32 and IL32 transcript variants (α, ß, γ and δ) in peripheral blood CD3+ cells from healthy controls and MDS patients.Methods:
CD3+ cells were isolated by immunomagnetic cell sorting from thirty-nine untreated MDS patients and twenty-nine healthy donors. Gene expression was evaluated by quantitative PCR. For statistical analysis, MannWhitney test, Kruskal-Wallis test with Dunns post test and Log-rank (Mantel-Cox) were used, as appropriate. A p value <0.05 was considered statistically significant.Results:
IL32 expression and IL32 transcript variants IL32α, IL32ß, IL32γ, and IL32δ, were similar in peripheral blood CD3+ cells from healthy donors and MDS patients. Increased IL-32α expression was an independent predictor for MDS disease progression by univariate and multivariate analysis.Conclusions:
We observed that IL32 expression is not differently expressed in CD3+ cells from MDS patients; nevertheless IL32α has a potential role in disease progression (AU)
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Syndromes myélodysplasiques
/
Analyse multifactorielle
/
Interleukines
/
Antigènes CD3
/
Évolution de la maladie
/
Système immunitaire
Type d'étude:
Étude pronostique
Limites du sujet:
Adulte
/
Adulte très âgé
/
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Appl. cancer res
Thème du journal:
Tumeurs
Année:
2017
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
University of Campinas, Hemocentro-Unicamp/BR
/
University of São Paulo at Ribeirão Preto Medical School/BR
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