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Detection of sputum cofilin-1 as indicator of malignancy
Rangel, M P; Antonangelo, L; Acencio, M M P; Faria, C S; de Sá, V K; Leão, P S; Farhat, C; Fabro, A T; Longatto Filho, A; Reis, R M; Takagaki, T; Capelozzi, V L.
  • Rangel, M P; Universidade de São Paulo. Faculdade de Medicina. Departmento de Patologia. São Paulo. BR
  • Antonangelo, L; Universidade de São Paulo. Faculdade de Medicina. Departmento de Patologia. São Paulo. BR
  • Acencio, M M P; Universidade de São Paulo. Instituto do Coração (Incor). Divisão de Pneumologia. São Paulo. BR
  • Faria, C S; Universidade de São Paulo. Faculdade de Medicina. Laboratório de Investigação Médica (LIM 03). São Paulo. BR
  • de Sá, V K; Universidade de São Paulo. Faculdade de Medicina. Departmento de Patologia. São Paulo. BR
  • Leão, P S; Universidade de São Paulo. Instituto do Coração (Incor). Divisão de Pneumologia. São Paulo. BR
  • Farhat, C; Universidade de São Paulo. Faculdade de Medicina. Departmento de Patologia. São Paulo. BR
  • Fabro, A T; Universidade de São Paulo. Departmento de Patologia. Ribeirão Prêto. BR
  • Longatto Filho, A; Universidade de São Paulo. Faculdade de Medicina. Laboratório de Investigação Médica (LIM 14). São Paulo. BR
  • Reis, R M; Fundação Pio XII. Hospital de Câncer de Barretos. Centro de Pesquisa em Oncologia Molecular. Barretos. BR
  • Takagaki, T; Universidade de São Paulo. Instituto do Coração (Incor). Divisão de Pneumologia. São Paulo. BR
  • Capelozzi, V L; Universidade de São Paulo. Faculdade de Medicina. Departmento de Patologia. São Paulo. BR
Braz. j. med. biol. res ; 51(8): e7138, 2018. tab, graf
Article Dans Anglais | LILACS | ID: biblio-951750
ABSTRACT
Cofilin-1 (CFL1), a small protein of 18 kDa, has been studied as a biomarker due to its involvement in tumor cell migration and invasion. Our aim was to evaluate CFL1 as an indicator of malignancy and aggressiveness in sputum samples. CFL1 was analyzed by ELISA immunoassay in the sputum of 73 lung cancer patients, 13 cancer-free patients, and 6 healthy volunteers. Statistical analyses included ANOVA, ROC curves, Spearman correlation, and logistic regression. Sputum CFL1 levels were increased in cancer patients compared to cancer-free patients and volunteers (P<0.05). High expression of sputum CFL1 was correlated to T4 stage (P=0.01) and N stage (P=0.03), tobacco history (P=0.01), and squamous cell carcinoma histologic type (P=0.04). The accuracy of sputum CFL1 in discriminating cancer patients from cancer-free patients and healthy volunteers were 0.78 and 0.69, respectively. CFL1 at a cut-off value of 415.25 pg/mL showed sensitivity/specificity of 0.80/0.70 in differentiating between healthy volunteers and cancer patients. Sputum CFL1 was also able to identify cancer-free patients from patients with lung cancer. The AUC was 0.70 and, at a cut-off point ≥662.63 pg/mL, we obtained 60% sensitivity and 54% specificity. Logistic regression analysis controlled for tobacco history, histologic types, and N stage showed that cancer cell-associated CFL1 was an independent predictor of death. Smoker patients with squamous cell carcinoma, lymph node metastasis and sputum CFL1>1.475 pg/mL showed augmented chance of death, suggesting lung cancer aggressiveness. CFL1 presented diagnostic value in detecting lung cancer and was associated to tumor aggressiveness.
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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Expectoration / Carcinome épidermoïde / Marqueurs biologiques tumoraux / Cofiline-1 / Tumeurs du poumon Type d'étude: Etude diagnostique / Étude observationnelle / Étude pronostique / Facteurs de risque Limites du sujet: Adulte / Adulte très âgé / Femelle / Humains / Mâle langue: Anglais Texte intégral: Braz. j. med. biol. res Thème du journal: Biologie / Médicament Année: 2018 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Fundação Pio XII/BR / Universidade de São Paulo/BR

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Texte intégral: Disponible Indice: LILAS (Amériques) Sujet Principal: Expectoration / Carcinome épidermoïde / Marqueurs biologiques tumoraux / Cofiline-1 / Tumeurs du poumon Type d'étude: Etude diagnostique / Étude observationnelle / Étude pronostique / Facteurs de risque Limites du sujet: Adulte / Adulte très âgé / Femelle / Humains / Mâle langue: Anglais Texte intégral: Braz. j. med. biol. res Thème du journal: Biologie / Médicament Année: 2018 Type: Article Pays d'affiliation: Brésil Institution/Pays d'affiliation: Fundação Pio XII/BR / Universidade de São Paulo/BR