LPS levels in root canals after the use of ozone gas and high frequency electrical pulses
Braz. oral res. (Online)
;
30(1): e19, 2016. tab, graf
Article
Dans Anglais
| LILACS
| ID: biblio-951989
ABSTRACT
Abstract The present study aims to verify the effect of ozone gas (OZY® System) and high frequency electric pulse (Endox® System) systems on human root canals previously contaminated with Escherichia colilipopolysaccharide (LPS). Fifty single-rooted teeth had their dental crowns removed and root lengths standardized to 16 mm. The root canals were prepared up to #60 hand K-files and sterilized using gamma radiation with cobalt 60. The specimens were divided into the following five groups (n = 10) based on the disinfection protocol used OZY® System, one 120-second-pulse (OZY 1p); OZY® System, four 24-second-pulses (OZY 4p); and Endox® System (ENDOX). Contaminated and non-contaminated canals were exposed only to apyrogenic water and used as positive (C+) and negative (C-) controls, respectively. LPS (O55B55) was administered in all root canals except those belonging to group C-. After performing disinfection, LPS samples were collected from the canals using apyrogenic paper tips. Limulus Amoebocyte Lysate (LAL) was used to quantify the LPS levels, and the data obtained was analyzed using one-way ANOVA. The disinfection protocols used were unable to reduce the LPS levels significantly (p = 0.019). The use of ozone gas and high frequency electric pulses was not effective in eliminating LPS from the root canals.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Oxydants photochimiques
/
Ozone
/
Lipopolysaccharides
/
Cavité pulpaire de la dent
/
Électrocoagulation
/
Endotoxines
/
Escherichia coli
Type d'étude:
Essai clinique contrôlé
/
Études d'évaluation
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Braz. oral res. (Online)
Thème du journal:
Dentisterie
Année:
2016
Type:
Article
Pays d'affiliation:
Brésil
/
Royaume-Uni
Institution/Pays d'affiliation:
Pontifícia Universidade Católica do Rio Grande do Sul/BR
/
University of Warwick/GB
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