Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection
Rev. Soc. Bras. Med. Trop
;
51(4): 467-474, July-Aug. 2018. graf
Article
Dans Anglais
| LILACS
| ID: biblio-957441
ABSTRACT
Abstract INTRODUCTION:
The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry (IHC) may serve as a promising strategy. Here, we validate an antiserum for the detection of T. cruzi infection by IHC.METHODS:
We used 1) positive controls (PCs) - 13 EMB, 12 skin biopsies, and 1 heart with T. cruzi nests as sections stained with hematoxylin and eosin (HE); 2) negative controls - a) 10 explant hearts and 10 EMB with no amastigote nests or clinical/laboratory signs of chagasic infection; and b) eight samples with leishmaniasis, toxoplasmosis, or histoplasmosis; and 3) Cases - 31 EMB of chagasic patients with no parasite nests in HE sections but detected positive for T. cruzi DNA by polymerase chain reaction. As a primary antibody, a hyperimmune serum from T. cruzi-infected rabbits was used.RESULTS:
IHC results were positive for 21 of 26 PCs (80.8%) and one case of cutaneous leishmaniasis. In 4 of 31 cases, IHC revealed nests (12.9%), which were undetected by conventional histological examination.CONCLUSIONS:
This study shows that IHC with the tested antiserum increases the sensitivity of the diagnosis and may be recommended for routine use in EMB analyses of cardiac transplant patients with Chagas disease.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Sujet Principal:
Trypanosoma cruzi
/
Anticorps antiprotozoaires
/
ADN des protozoaires
/
Maladie de Chagas
/
Endocarde
/
Anticorps monoclonaux
Type d'étude:
Etude diagnostique
/
Étude observationnelle
/
Étude pronostique
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Rev. Soc. Bras. Med. Trop
Thème du journal:
Médecine tropicale
Année:
2018
Type:
Article
Pays d'affiliation:
Brésil
Institution/Pays d'affiliation:
Universidade Federal de Minas Gerais/BR
/
Universidade Federal de Ouro Preto/BR
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