Isolation and Identification of Novel IndigenousBacterial Strain as a Low Cost Pectinase Source
Braz. arch. biol. technol
;
61: e18160653, 2018. graf
Article
Dans Anglais
| LILACS
| ID: biblio-974066
ABSTRACT
ABSTRACT The present study was concerned with the searching of novel bacterial cultures from different samples for the lab scale production of pectinase. Keeping in view the increasing demand of pectinase specially in Faisalabad, an industrial city of Pakistan, isolation of new hyper producer bacterial strains locally is an easy and cheap way of getting the desirable products at low cost. Therefore, isolation of new strains for industrial enzyme production has been and will be remained a part of research every time. This method alone can also provide raw material for further research such as enzyme engineering or molecular directed evolution. For the identification of hyper producer strain colony PCR was done for 16S rRNA analysis. Reason to use the 16S rRNA for identification purpose is that the gene is fairly short and can be amplified quickly and easily. The bacterial isolate (sources of pectinase enzyme) was identified based on PCR amplification of 16S rRNA and for this purpose the amplified product was run in agarose gel against a known species of Bacillus licheniformis. The 16S rRNA sequencing confirmed the Bacillus status of the strain.
Texte intégral:
Disponible
Indice:
LILAS (Amériques)
Type d'étude:
Etude diagnostique
/
Évaluation en économique de la santé
langue:
Anglais
Texte intégral:
Braz. arch. biol. technol
Thème du journal:
Biologie
Année:
2018
Type:
Article
Pays d'affiliation:
Pakistan
Institution/Pays d'affiliation:
GC University/PK
/
National Institute for Biotechnology and Genetic Engineering/PK
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS