Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;52(2): e8194, 2019.
Article
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| ID: biblio-984032
Bibliothèque responsable:
BR1.1
ABSTRACT
Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells (≤7×103/µL) in the sample. High and low cellularity in BM and high and low cellularity in PB samples showed no metaphases in 3.9, 39.7, 41.9, and 84.6% of cases, respectively. Collecting a good BM sample is the key for the success of karyotyping in MN and avoids the use of expensive molecular techniques.
Mots clés
Texte intégral:
1
Indice:
LILACS
Sujet Principal:
Manipulation d'échantillons
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Syndromes myélodysplasiques
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Cellules de la moelle osseuse
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Leucémie myéloïde
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Caryotypage
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Syndromes myéloprolifératifs
Type d'étude:
Diagnostic_studies
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Risk_factors_studies
Limites du sujet:
Adolescent
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Adult
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Aged
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Aged80
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Female
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Humans
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Male
langue:
En
Texte intégral:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Thème du journal:
BIOLOGIA
/
MEDICINA
Année:
2019
Type:
Article