Ultrasound effects on lens protein and its membrane of rabbits' eye

ABSTRACT

In the present work, the effect of ultrasound [US] therapeutic doses on the rabbit's eye lens protein and its membrane was studied. The characteristics of eye lens protein are studied at the power intensities of 0.5 and 3 W/cm[2] at frequencies of 2.8, 4.5 and 10.8 MHz for exposure durations 10, 20 and 40 minutes. All the studied groups have their own controls. Na [+] - K[+] ATPase activity of the eye lens membrane, total soluble protein and electrophoretic fractionation for the eye lens proteins were carried out for the studied groups. The obtained results showed that there is a noticeable decrease in ATPase activity which was a function of power intensity, frequency and duration of US exposure which reached to -38.37% at the power intensity 3W/cm[2], at frequency 10.8 MHz and exposure time of 40 minutes. Also, total protein content decreased by increasing the power intensity, duration of US exposure and the frequency. It reaches -11.1% at the power intensity 3 W/cm[2], frequency 10.8 MHz and duration of exposure of 40 minutes. The electorphoretic separation indicated completely change after US sonification with different used power intensities at different frequencies and exposure times. Electrophoretic patterns showed a decrease in the intensity of high mobile fractions accompanied by an increase in electrophoretic mobility of low mobile ones. The patterns showed also an aggregation of lens proteins of low mobile fractions after exposure to 10and20minutes but at 40 minutes, there was an increase in the mobility of all fractions. .In addition, electrophoretic patterns showed a remarkable shift of all fractions, after 20 and 40 minutes of exposure to US towards low molecular weights. The safty therapeutic doses of US is at short time [less than 20 minutes], with low power intensity[0.5 W/cm[2] and low frequency .There is also recommendations for antioxidants supplementation for those treated with US