Studies on DNA sequencing of K-ras-2 Gene in patients with liver and pancreas cancer

ABSTRACT

Point mutations in K-ras-2 gene were detected either in primary liver or pancreas cancer patients using polymerase chain reaction followed by polyacrylamide DNA sequencing determination analysis. The frequencies of point mutations which found liver tumors were mainly due to adenine base insertion, whereas in pancreatic tumors cytosine insertion type was found to be abundant. Both types of tumors showed guanine deletion of K-ras-2 mutations. This work shed light on the possibility of using point mutations in K-ras-2 gene as a useful approach for differential diagnosis of cancers