Radioresponse of human lymphocytes pretreated with boron and gadolinium as assessed by the comet assay

ABSTRACT

Boron and gadolinium are among the nuclides that hold a unique property of being a neutron capture therapy agent. Neutron beams have often a considerable portion of gamma rays with fast neutrons. Gamma rays, as beam contaminants, can cause considerable damage to normal tissues even if such tissues do contain high boron concentrations. The modification of radioresponse in human lymphocytes pretreated with boron or gadolinium compound was studied by assessing the DNA damage using single cell gel electrophoresis [SCGE], the comet assay. The lymphocytes from the human peripheral blood were irradiated with 0, 1, 2 and 4 Gy of gamma rays from a 60Co isotopic source with or without pretreatment of boron or gadolinium compound for 10 minutes at 4°C. Post-irradiation procedures included slide preparation, cell-lysing, unwinding and electrophoresis, neutralization, staining, and analytic steps, gel electrophoresis. The results indicate that pretreatment with boron compound [50 nM or 250 nM of 10B] is effective in reducing the radiosensitivity of the lymphocyte DNA. Conversely, pretreatment with gadolinium compound [50 nM] led to a dose-dependent increase in the radiosensitivity, most prominently with a dose of 4 Gy [P<0.001]. Furthermore, when the lymphocytes were pretreated with a combined mixture [11] of boron [250 nM] and gadolinium [50 nM] compounds, the reduced radiosensitivity was also observed