[Comparison and evaluation of Echinococcus granulosus protoscoleces excretory/secretory proteins in PBS complemented with glucose, DMEM and RPMI culture media]
Journal of Zanjan University of Medical Sciences and Health Services. 2011; 19 (74): 44-53
Dans Persan
| IMEMR
| ID: emr-106555
ABSTRACT
Preparation of proper antigens is an important issue in serology of hydatidosis. Investigators have been able to obtain excretory/secretory antigens [E/S Ags] by short-term culture of protoscoleces in a couple of culture media. However, no data are available about production rate of such antigens in different culture media. The present study was carried out to evaluate the production of E/S Ags [proteins] in PBS complemented with glucose, DMEM and RPMI culture media. To obtain E/S proteins, protoscoleces of echinococcus were cultured in PBS complemented with 10% glucose, RPMI and DMEM for 72 hours. Proteins secreted in culture media were concentrated and assayed. To characterize different components, proteins were electrophoresed on SDSPAGE. Data were analyzed using One-way ANOVA and Tukey HSD tests. The mean concentration of E/S proteins in PBS medium in 24 hours of culture was significantly higher than DMEM and RPMI [P<0.05]. However, such a difference was not observed between E/S proteins in DMEM and RPMI media. E/S proteins obtained from PBS medium were separated into 12 major bands and the two other media into 14 major bands within a range of molecular masses of 16 to 67 kDa. PBS complemented with glucose is more appropriate than the two other media for E/S proteins production. The best time to obtain E/S proteins is the first 48 hours of culture
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Indice:
Méditerranée orientale
Sujet Principal:
Tests sérologiques
/
Protéines
/
Analyse de variance
/
Milieux de culture
/
Échinococcose
/
Échinococcose hépatique
/
Antigènes d'helminthe
langue:
Persan
Texte intégral:
J. Zanjan Univ. Med. Sci. Health Serv.
Année:
2011
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