Identification of apoptosis in rat ameloblast in different stages via specific labeling of DNA fragmentation

Apoptosis is associated with physiological or programmed cell death, in contrast with necrosis, which is associated with cell injury. The apop tag plus labeling method is more specific for apoptotic cells using paraffin section of the ameloblasts in rats. The results of the present study revealed that most apoptotic cells were adequately labeled showing apoptotic bodies of various sizes, cells shrinkage, no inflammatory response, phagocytosis by adjacent normal cells cytoplasmic vacuolation preservation of plasma membrane integrity. Therefore, an important application is the quantification in tumors, in which apoptosis is difficult to assess