Cloning of coagulant VII factor from hepatoma cell line
Journal of Research in Medical Sciences. 2010; 34 (1): 20-25
Dans Persan
| IMEMR
| ID: emr-108606
ABSTRACT
Factor VII, is a coagulant protease; it begins the proteolytic cascade reactions and produces thrombin. The use of recombinant human factor VII, [rhFVII] is effective for the treatment of patients with hemophilia A or B. It is a target for gene therapy. This study was done to clone factor VII from HepG2 cell line. RNA was extracted from the hepatoma, [HepG2], cell line. On reverse transcription FVII cDNA was amplified by RT-PCR. PCR product was cloned into the pTZ57R/T vector and transported into the E-coli cells. By amplification of the FVII gene, the PCR band was observed and cloning into the vector was confirmed by restriction analysis. In this paper we report the cloning of factor VII from HepG2 cell line
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Indice:
Méditerranée orientale
Sujet Principal:
Thérapie génétique
/
Réaction de polymérisation en chaîne
/
Hémophilie B
/
Clonage moléculaire
/
Carcinome hépatocellulaire
/
RT-PCR
/
Hémophilie A
langue:
Persan
Texte intégral:
J. Res. Med. Sci.
Année:
2010
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