Infectious bursal disease virus infection among Egyptian poultry flocks 1- Detection and isolation of the virus

ABSTRACT

A total of 46 suspected bursae have been collected from different governorates in Egypt. These samples represented Dakahlia, Ismailia, Fayoum, Sharkia, Giza and Kaliobia [Banha] 12, 10, 10, 4, 5, 5, respectively. These bursae have been firstly tested by the AGPT, using specific chicken anti-IBDV serum, for the presence of specific Gumboro viral antigen [s] before being subjected to viral isolation. Gumboro virus was isolated from every infected bursa that was giving positive reaction in the AGPT, using SPF-9 days old embryonated chicken eggs [ECE], chicken embryo fibroblast [CEF] and QT-35 cells