Identification rapide de streptococcus pyogenes serotype M12 dans les prelevements de gorge part coagglu tination extemporanee

ABSTRACT

A rapid procedure for identification of Streptococcus pyogenes serotype M. 12 directly in throat swabs, is reported and compared with standard culture method on blood agar plates and typing of group A Streptococci isolated with double gel immuno-diffusion This procedure consist of chlorhydric acid extraction of swabs and testing of the extract towards specific M. 12 protein serum using extemporaneous coagglutination technique. We have tested 1100 throat swabs, with this procedure and with standard culture Procedure Identification of group A Streptococci serotype M. 12 with reported method is obtainable within 30 to 45 minutes of receipt of the clinical specimen. This method is easy to perform with a sensitivity and a specificity respectively 89.7% and 98,8%