Bacterial sensitivity in neonatal sepsis

ABSTRACT

To determine the bacterial sensitivity and resistance patterns to different antibiotics in neonatal sepsis. A descriptive study including indoor patients [neonates] of Department of Neonatology, Children's Hospital, Pakistan Institute of Medical Sciences, Islamabad from March, 2009 to September, 2009. A structured Performa was used to collect the information for the baseline characteristics like age, gender, birth weight, gestational age, mode of delivery of the neonate and age of onset of illness. The sensitivity and resistance patterns were checked by standard disc diffusion method of all the organisms found with Ampicillin, Cefotaxime, ceftazidime, ceftriaxone, Amikacin, imipenam, Ofloxacin, Azetreonam and in some cases Vancomycin. The collected data was analyzed by using SPSS version 12.0. A total of 50 Neonates with blood culture proven neonatal sepsis were included in the study by consecutive sampling. 27[54%] children presented with late onset neonatal sepsis and 23 [46%] with early neonatal sepsis. The most common organism was Enterobactor [48%] followed by Escherichia coli [16%] and Klebsiella [14%]. The highest resistance of Staphylococcus aureus was with cefotaxime [80%] followed by Enterobactor [58%]. Majority of the organisms were resistant for ceftazidime [100% by Staphylococcus, 79.2% by Enterobactor, 75% by Escherichia coli and 71% by klebsiella]. Ceftriaxone was found highly resistant for most of the organism's i.e. Klebsiella [71.4%], Enterobactor [70.8%], Staphylococcus aureus [60%] and Escherichia coli [50%]. The sensitivity of the Staphylococcus with these antibiotics was almost zero, only with vancomycin with which it was 100% sensitive. Most common organisms were Enterobactor [48%] followed by Escherichia coli [16%] and Klebsiella [14%]