Discrimination of entamoeba spp. in children with dysentery
Gastroenterology and Hepatology from Bed to Bench. 2014; 7 (3): 164-167
Dans Anglais
| IMEMR
| ID: emr-147111
ABSTRACT
The present study was performed in order to differentiate E. histolytica and E. dispar in children from Gorgan city, using a PCR method. Differential detection of two morphologically indistinguishable protozoan parasites Entamoeba histolytica and E. dispar has a great clinical and epidemiological importance because of potential invasive pathogenic E. histolytica and non-invasive parasite E. dispar. One hundred and five dysentery samples were collected from children hospitalized in Taleghani hospital in Gorgan city. The fecal specimens were examined by light microscopy [10X then 40X] to distinguish Entamoeba complex. A single round PCR amplifying partial small-subunit rRNA gene was performed on positive microscopy samples to differentiate E. histolytica/ E. dispar and E.moshkovskii from each other. Twenty-five specimens [23.8%] were positive for Enramoeba complex in direct microscopic examination. PCR using positive controls indicated E. histolytica and E. dispar in two [2/25, 8%] and three [3/25, 12%] samples, respectively. There is a warrant to performing molecular diagnosis for stool examination at least in hospitalized children in order to prevent incorrect reports from laboratories and consequently mistreating by physicians
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Indice:
Méditerranée orientale
langue:
Anglais
Texte intégral:
Gastroenterol. Hepatol. Bed Bench
Année:
2014
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