[Evaluation of 4-Amino salicylic acid chelating effect on healing of manganese induced intoxications in male Wistar rat's liver]

ABSTRACT

Manganese related jobs [Mn miners] may cause Manganism, a Parkinson like disease. Chelators are suggested to reduce these effects. This study was carried out to investigate the effect of 4-Amino Salicylic acid chelating effects. This experimental study was done on 20 adult male Wistar rats. The rats were classified into four groups, 5 in each group, as follows one control and three experimental groups. In two phases the groups received Salin-Salin, Manganese-Salin, Salin-4-Amino acid and Manganese -4-Amini acid, respectively. Eight mg/kg of manganese chloride i.p. injection for 1 week and 1.5g/l of PAS s.c injection five-day in a week for four weeks, were used for intoxication and treatment, respectively. Serum and liver tissues were separated. Data were analyzed using ANOVA and Tukey's tests. Biochemical factors showed cholesterol increment in group 2 compared with group 1 and decrement of cholesterol level in the blood of group 4 compare with group 2 [P<0.01]. Also increasing the direct billirubin in the Mn and PAS groups was more significant compared with control group [P<0.05]. Billirubin was observed in the urine of the Mn, PAS, Mn+PAS groups and urobilinogen declining was decreased in the urine of these groups [P<0.05]. There was a significant increase [P<0.05] of OT, PT enzymes in the serum of PAS and ALP in the Mn and Mn+PAS groups compared with control group. PAS can play a significant role in reducing destructive effects of Mn, especially on liver but has different side effects, like other chemical drugs