[Detection of somatic antigens in Mycobacterium avium subspecies paratuberculosis]

ABSTRACT

Johne's disease or Paratuberculosis as a chronic granulomatosis enteritis in ruminants will be caused by Mycobacerium avium subsp . Paratuberculosis . Detecting whole bacterial cell wall antigens would be helpful in potential applications for diagnosis, vaccine production, and elucidation of pathogenesis . To determine secreted somatic cell antigens of Mycobacterium avium subspecies Paratuberculosis . Standard strain [III-V] of Mycobacterium avium subspecies Paratuberculosis DNA was extracted from the cultured and gene analysis was done using PCR to confirm bacterial purity . On the other hand, protein concentrations in both media and cell entracts were determined . Furthermore, all proteins pattern were shown by SDS -PAGE . Electrophoretic findings showed some somatic antigens in the range of 19 - 100 KDa. These purified somatic antigens can be used for further study and potential application in vaccine production