Differentiation potential of O Bombay human-induced pluripotent stem cells and human embryonic stem cells into fetal erythroid-like cells
Cell Journal [Yakhteh]. 2015; 16 (4): 426-439
Dans Anglais
| IMEMR
| ID: emr-154845
ABSTRACT
There is constant difficulty in obtaining adequate supplies of blood components, as well as disappointing performance of "universal" red blood cells. Advances in somatic cell reprogramming of human-induced pluripotent stem cells [hiPSCs] have provided a valuable alternative source to differentiate into any desired cell type as a therapeutic promise to cure many human disease. In this experimental study, we examined the erythroid differentiation potential of normal Bombay hiPSCs [B-hiPSCs] and compared results to human embryonic stem cell [hESC] lines. Because of lacking ABO blood group expression in B-hiPSCs, it has been highlighted as a valuable source to produce any cell type in vitro. Similar to hESC lines, hemangioblasts derived from B-hiPSCs expressed approximately 9% KDR+CD31+ and approximately 5% CD31+CD34+. In semisolid media, iPSC and hESC-derived hemangioblast formed mixed type of hematopoietic colony. In mixed colonies, erythroid progenitors were capable to express CD71+GPA+HbF+ and accompanied by endothelial cells differentiation. Finally, iPS and ES cells have been directly induced to erythropoiesis without hemangioblast formation that produced CD71+HbF+erythroid cells. Although we observed some variations in the efficiency of hematopoietic differentiation between iPSC and ES cells, the pattern of differentiation was similar among all three tested lines
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Indice:
Méditerranée orientale
Sujet Principal:
Cellules érythroïdes
/
Cellules souches embryonnaires
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Cell J. [Yakhteh]
Année:
2015
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