Inhibition of luminol-dependent chemiluminescence of human granulocytes by low doses of inorganic mercury
EMHJ-Eastern Mediterranean Health Journal. 2000; 6 (2-3): 425-431
Dans Anglais
| IMEMR
| ID: emr-157814
ABSTRACT
HgCl2, added in vitro to human granulocytes in whole blood, caused a marked inhibitory effect on the luminol-dependent chemiluminescence induced by BaS04 crystals in suspension of these cells. The effect was both dose- and time-dependent when BaS04 was used to stimulate the oxidative burst in granulocytes. Incubation with the highest concentration of HgCl2 used [10 mmol/L], however, did not cause disruption of the membranes of granulocytes. The effect of HgCl2 on the granulocytes was irreversible following washing of the HgCl2-treated cells with phosphate buffered saline. HgCl2 did not affect chemiluminescence produced when luminol was excited by oxidative hydrogen peroxide in a cell-free medium. These results suggest that some of the toxicity of HgCl2 may be greater than mediated by an action on the phagocytic immune system
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Indice:
Méditerranée orientale
Sujet Principal:
Composés du mercure
/
Granulocytes
/
Mesures de luminescence
/
Luminol
langue:
Anglais
Texte intégral:
East Mediterr Health J.
Année:
2000
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