Pentoxifylline protects against carbon tetrachloride induced liver injury in adult male wistar rat model

ABSTRACT

To investigate the protective effect of Pentoxifylline [PTX] in carbon tetrachloride [CC1[4]] induced liver injury in adult male Wistar rat model. Experimental/Analytical study. Animal House, Isra University Hyderabad from May to December 2012. Forty five adult male Wistar rats were divided into three groups; Group A. controls received 0.9% isotonic saline, Group B. received CC1[4] orally [I.9mg/kg] mixed in olive oil, and Group C. received the PTX+ CC1[4]. Blood samples were collected for liver biochemical assays. The animals were sacrificed, liver tissue, after fixation in 4% formaldehyde, was embedded in paraffin. Tissue sections of 5micro thickness were subjected to haematoxylin and eosin staining and were assessed by light microscopy. The data was analyzed on SPSS 21.0 using one-way ANOVA, Tukey-Cramer and Chi-square tests. A p-value of liver biochemical and histological findings reveal statistically significant differences among the controls, CC1[4] and PTX+ CC1[4] groups [p=0.0001]. Liver enzymes and histology was deranged significantly in CC1[4] group compared to controls and PTX+ CC1[4] group [p=0.0001]. The CC1[4]+PTX group shows less elevation of liver enzymes and derangement in liver histology when compared to CC1[4] group [p=0.001]. The histological findings of congestion, inflammatory cell infiltrate, vacuolar degeneration and necrosis are found prominent in CC1[4] group. The Pentoxifylline protects against oxidative damages caused by carbon tetrachloride induced liver injury in rat model