Isolation and molecular identification of klebsiella microbe isolated from chicks

ABSTRACT

Klebsiella pneumoniae is one of the most common infectious disease affecting chicks, causing great economic losses. Also, possess food safety and antimicrobial resistance threats, as it may act as a contamination source of poultry meat and eggs. We aim to isolation and identification of klebsiella organisms causing death and loss in chicks. To perform this aim, it is necessary to full following points Isolation of klebsiella causing respiratory manifestations and death in chicks purification and identification [morphologically, culturally, biochemically] of recovered klebsiella, antibiotic sensitivity test, PCR detection of Klebsiella genus specific gene [GyrA], PCR detection of klebsiella carbapenem resistance gene [kpc] and PCR detection of two of important virulence genes ; Mucoviscocity attached gene [MagA] and Iron uptake system gene [Kfu]. A total of 150 chicks from different locations were examined clinically for respiratory symptoms lung, liver, trachea, intestine and samples [dropping and oro-pharyngeal swab] were collected for bacteriological examination. The results of biochemical tests for detection of biochemical characteristics of isolated Klebsiella where all the examined isolates cleared that Klebsiella, gave positive reaction for catalase test, voges proskeure, urease and citrate test, meanwhile the isolates were negative for oxidase, Indole and Methyl red tests. Cultivation of isolate on macConkey gives lactose fermenting colonies, more or less dome shaped,3-4 mm diameter after overnight incubation at 37C .The sixteen klebsiella isolates subjected for PCR detection and the results for detection of Klebsiella genus specific gene [GyrA] were positive for all isolates. Also ten of sixteen subjected to PCR for detection of Klebsiella pneumonia carbapnamase gene [KPC] and the results were negative, ten of sixteen subjected to PCR for detection,of Mucoviscocity attached gene [Mag A] and the results were positive for seven out of ten isolates,eight out of sixteen isolates subjected to PCR for detection of Iron uptake system gene [Kfu] and the results were positive for eight out of ten isolates. The 16 Klebsiella isolates were tested for the resistance to antibiotics and the results indicates that Meropenem, Impienem, Amikacin, Cefotaxime sodium, Ceftazidime, Ciproflaxin and Tobramycin had high effect on klebsiella isolates. Meanwhile, Gentamycin and Cefepime have moderate effect on Klebsiella . Moreover, antibiotics have less effect on the isolated Klebsiella were Azithromycin and Erythromycin