possible role of Petroselinum Sativum extract against oxidative stress in CCL[4]-induced hepatotoxicity in albino rats

ABSTRACT

The present study investigated the possible effect of the ethanolic extract of Petroselinum sativum against CCl[4]-induced hepatotoxicity in rats and compared with Rutin as reference. Ethanolic extract of Petroselinum sativum in two concentrations 100 and 200 mg/kg.bw. Was orally administered for 21 days after i.p CCl[4] injection to evaluate its possible effect. Also, Rutin [as standard antioxidant] was orally administered by concentration of 200 mg/kg.bw. Malondialdehyde "MDA" concentration [the end product of lipid peroxide] and total protein were determined both in liver, homogenate and plasma. Reduced Glutathione "GSH" content was determined in liver homogenate and in whole blood. Albumin was also determined and Glutathione-S-transferase "GST" in serum of rats was performed. Histological studies of liver for all treatments were investigated and results revealed that, the lipid peroxides "MDA" and "GST" were significantly increased [p<0.05], while reduced glutathione was decreased after treatment of CCL[4]. Administration of Petroselinum Sativum ethanolic extract at two concentrations and Rutin exerted a significant decreases [p<0.05] in MDA concentration, while the reduced glutathione appeared to be increased in whole blood. The activity of GST was reduced due to the effect of Petroselinum Sativum and Rutin after the duration time [10 and 21 days]. The ethanolic extract of Petroselinum Sativum revealed a significant increase in the total protein, albumin, and A/G ratio. The histological studies showed a significant increase in liver injury of intoxicated rats. After treatment with ethanolic extract of Petroselinum Sativum as well as Rutin, the sites of injury were decreased. It was concluded that, the toxic effect of CCl[4] on liver was abrogated under the effect of Petroselinum Sativum ethanolic extract; also, Rutin had an inhibitory effect on lipid peroxidation. These findings suggested that the plant extract of Petroselinum Sativum could has a potent antioxidant activity