effect of stressful exercise and / or local implants of labeled Buffy coat cells on muscle regeneration

ABSTRACT

The process of muscle regeneration is an important field in scientific work Thus. An attempt has been carried out to find the link between the most important factors which may affect this process and these are exercise and local buffy coat implantation. 168 male albino rats were divided into 5 groups. All of these groups were exposed to unilateral calf muscle injury of their right legs. The 1[st] group was regarded as control, the 2[nd] was exposed to stressful exercise, the 3[rd] was exposed to local implantation of labelled autogenous buffy coat cells at the site of muscle injury, the 4[th] was exposed to both events while the 5[th] was exposed to local injection of the labelling material at the injury site. Follow up of the process of muscle regeneration had been carried out for 28 days. Results have shown that muscle regeneration was enhanced in the 2[nd] and 4[th] groups where stressful exercise had been performed, possibly by providing myoblast precursors from the circulation and / or increasing myoblast spillage into the area by multiple fiber disruptions . Buffy coat had also enhanced muscle regeneration by transformation of some of them into myoblasts as indicated by the presence of labelled cells in the regenerated muscle tissue in the 3[rd] and 4[th] groups. These findings had led to the conclusion that, muscle regeneration can be improved by using stressful muscle exercise together with local implantation of buffy coat cells at the site of muscle injury. An intimate relation had been predicted between buffy coat implantation, stressful exercise and muscle regeneration