Assessment of the plasminogen level in the peritoneal fluid as an indicator of ovulation in infertile women

ABSTRACT

Peritoneal fluid [PF] samples were collected from 60 infertile with regular menses at different time of spontaneous cycle. In 35 patients, the PF sample was collected via laparoscopy while in the remaining 25 patients, the PF sample was collected via culdocentesis. Radial immunodiffusion assay for the plasmingoen level in the collected PF samples was done. Dating of the endometrium was done for each patient in the same time. Twenty six patients were found to be in the proliferative phase while 34 patients were studied during the luteal phase. The luteal phase group was further subdivided into 3 subgroups based on histologic dating of the endometrium a] Early luteal phase group post ovulatory 1 - 4 days. B] Mid luteal phase group post ovulatory 5-9 days. c] Late luteal phase group post ovulatory 10-14 days. This study demonstrated that the plasminogen level in PF is significantly elevated shortly after ovulation [during the early luteal phase], reaching a level of mean [6.2 mg/d1] then declining gradually during the mid and late luteal phases. Its mean level in the preovulatory phase is much lower reaching 3.4 mg/d1