Application of multiplex PCR for Rapid and sensitive detection of human papillomaviruses in cervical cancer

ABSTRACT

Objectives: Reffering to an increase in cervical cancer in the recent years, rapid, sensitive and economical detection of human papillomaviruses [HPVs] as causative agents of cervical cancer is important. The traditional methods for the detection of HPVs in cervical cancer, such as pap smear, suffer from limitation and PCR has a potential to overcome the limitaitons. The purpose of present research work was to identify the five important strains of HPV [16, 18, 31, 33 and 45] simultaneously by Multiplex PCR application