[Construction of mutant WbkA gene in Brucella abortus S19 by overlap extension PCR]

ABSTRACT

Background: Causing site direct mutation can be one of the efficient methods to evaluate the characteristics and properties of various genes. Brucellosis is the most common zoonotic infectious disease that would cause great economic losses. Thus, recognition of pathogenic and immunogenic factors in the genus Brucella can lead to control this health problem: