Development of a single stranded DNA aptamer as a molecular probe for LNCap cells using Cell-SELEX

ABSTRACT

Background: Nowadays, highly specific aptamers generated by cell SELEX technology[systematic evolution of ligands by exponential enrichment] are being applied forearly detection of cancer cells. Prostate Specific Membrane Antigen [PSMA], over expressedin prostate cancer, is a highly specific marker and therefore can be used fordiagnosis of the prostate cancer cells. The aim of the present study was to select singlestrandedDNA aptamers against LNCap cells highly expressing PSMA, using cell-SELEX method which can be used as a diagnostic tool for the detection of prostatecancer cells