Inhibitory effects of some carbohydrates on nano-globular aggregation of both normal and glycated albumin

Background: Protein aggregation is one of the important, common and troublingproblems in biotechnology, pharmaceutical industries and amyloid-re-lated disorders

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Methods: In the present study, the inhibitory effects of some carbohydrates [alginate,beta -cyclodextrin and trehalose] on the formation of nano-globular aggregates fromnormal [HSA] and glycated [GHSA] human serum albumin were studied; when theformation of aggregates was induced by the simultaneous heating and addition ofdithiotheritol. For the investigations, the biophysical methods of UV-vis spectrophotometry,circular dichroism spectroscopy, transmission electron microscopy andtensiometry were employed

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Results: The effect of inhibitory mechanism of these inhibitors on the aggregation ofHSA and GHSA was expressed and compared together

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Conclusion: The results showed that the nucleus formation step of the aggregationprocess of HSA and GHSA was different in the presence of alginate [compared tobeta -cyclodextrin and trehalose]. The inhibition efficiencies of the carbohydrates on the aggregateformation of HSA and GHSA were different, arising from the differences inthe hydrophobicities of HSA and GHSA, and also, the differences between HSA-andGHSA-carbohydrate interactions