Rational development of a polycistronic plasmid with a CpG-free bacterial backbone as a potential tool for direct reprogramming
Cell Journal [Yakhteh]. 2017; 18 (4): 565-581
Dans Anglais
| IMEMR
| ID: emr-185782
ABSTRACT
Objective:
Induced pluripotent stem cells are generated from somatic cells by direct reprogramming. These reprogrammed pluripotent cells have different applications in biomedical fields such as regenerative medicine. Although viral vectors are widely used for efficient reprogramming, they have limited applications in the clinic due to the risk for immunogenicity and insertional mutagenesis. Accordingly, we designed and developed a small, non-integrating plasmid named pLENSO/Zeo as a 2A-mediated polycistronic expression vector
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Indice:
Méditerranée orientale
Sujet Principal:
Plasmides
/
Bactéries
/
Ilots CpG
/
Cellules souches pluripotentes induites
langue:
Anglais
Texte intégral:
Cell J. [Yakhteh]
Année:
2017
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