Histological and immunohistochemical study on the effect of noise stress on the spleen of adult albino rat and the possible role of Ginkgo biloba extract
Egyptian Journal of Histology [The]. 2013; 36 (3): 546-555
de En
| IMEMR
| ID: emr-187224
Bibliothèque responsable:
EMRO
Introduction: It is widely accepted that stress has an immunosuppressive effect. Ginkgo biloba extract has a broad spectrum of pharmacological properties
Aim: The aim of the study was to determine the effect of noise stress on the structure of the spleen and to detect the efficacy of G. biloba extract in counteracting these effects
Materials and methods: Thirty adult albino rats were divided equally into five groups. Group I was the control group; group II was the noise group, in which rats were exposed to noise for 4 weeks; and in group III [protected], rats were exposed to noise and received G. biloba [orally 100 mg/kg/day] from the beginning of the experiment for 4 weeks. In group IV [recovery], rats were exposed to noise for 4 weeks and then allowed a recovery period for another 4 weeks; in group V [treated], rats were exposed to noise for 4 weeks and then administration of G. bilobawas continued for another 4 weeks after stoppage of noise stress. At the end of the experiment, specimens from the spleen were taken and prepared for H and E and immunohistochemical stains [CD4 and CD68]
Result: In group II, there was a significant decrease in the size of the lymphatic follicles and many of them appeared to have focal areas of cellular depletions. There was a significant decrease in the number of both CD4+ T helper cells and CD68+ macrophages in comparison with the control group. In group III, a few follicles still showed small areas of cellular depletion. In groups IV and V [recovery and treated groups], a relative improvement in the structure of the spleen was observed, but a more or less complete improvement in group V was observed. However, the number of both CD4+ and CD68+ cells was still less than that in the control group
Conclusion: Noise stress had an immunosuppressive effect. Treatment with G. biloba was found to be more effective than its protective role
Aim: The aim of the study was to determine the effect of noise stress on the structure of the spleen and to detect the efficacy of G. biloba extract in counteracting these effects
Materials and methods: Thirty adult albino rats were divided equally into five groups. Group I was the control group; group II was the noise group, in which rats were exposed to noise for 4 weeks; and in group III [protected], rats were exposed to noise and received G. biloba [orally 100 mg/kg/day] from the beginning of the experiment for 4 weeks. In group IV [recovery], rats were exposed to noise for 4 weeks and then allowed a recovery period for another 4 weeks; in group V [treated], rats were exposed to noise for 4 weeks and then administration of G. bilobawas continued for another 4 weeks after stoppage of noise stress. At the end of the experiment, specimens from the spleen were taken and prepared for H and E and immunohistochemical stains [CD4 and CD68]
Result: In group II, there was a significant decrease in the size of the lymphatic follicles and many of them appeared to have focal areas of cellular depletions. There was a significant decrease in the number of both CD4+ T helper cells and CD68+ macrophages in comparison with the control group. In group III, a few follicles still showed small areas of cellular depletion. In groups IV and V [recovery and treated groups], a relative improvement in the structure of the spleen was observed, but a more or less complete improvement in group V was observed. However, the number of both CD4+ and CD68+ cells was still less than that in the control group
Conclusion: Noise stress had an immunosuppressive effect. Treatment with G. biloba was found to be more effective than its protective role
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Indice:
IMEMR
Sujet Principal:
Rats
/
Rate
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Immunohistochimie
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Extraits de plantes
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Résultat thérapeutique
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Agents protecteurs
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Ginkgo biloba
langue:
En
Texte intégral:
Egypt. J. Histol.
Année:
2013