Vitrification increased vacuolization of human spermatozoa: application of MSOME technology

ABSTRACT

Background: sperm vitrification is a technique of ice and cryoprotectant free cryopreservation by direct plunging of sperm suspension into liquid nitrogen [LN2]. The aim of this study was to investigate the influence of cryoprotectant free-vitrification on human sperm fine structure by MSOME technology and the fertility potential by zona binding assay [ZBA]