Determination of carbapenem resistance mechanism in clinical isolates of Pseudomonas aeruginosa isolated from burn patients, in Tehran, Iran
Journal of Epidemiology and Global Health. 2017; 7 (3): 155-159
Dans Anglais
| IMEMR
| ID: emr-188639
ABSTRACT
Carbapenems are the most important therapeutic options that effect against serious infections caused by multidrug resistant Pseudomonas aeruginosa [MDR-PA] isolates. Carbapenems resistant isolates of P. aeruginosa are increasing worldwide. The aim of this study was to determine the carbapenem resistance mechanisms in clinical P. aeruginosa isolates from burn patients, in Tehran, Iran. A total of 53 non-duplicated isolates of carbapenem-resistant P. aeruginosa were collected from burn patients. The presence of carbapenemase genes were determined by PCR. AmpC overproducer isolates were detected by phenotypic method. The mutation and transcription level of oprD were determined by PCR-sequencing and quantitative Real-time PCR [RT-PCR], respectively. Twenty-seven [50.9%] isolates were positive for carbapenemase [bla[vm] = 25 and bla[mp] = 2] and showed high-level resistance to imipenem and merope-nem. Twenty-eight isolates were AmpC overproducers. All isolates had a mutation in the oprD gene and down-regulation of oprD was found in 56.6% of MDR-PA isolates. Although the presence of carbapenemase is the common mechanism of resistant to carbapenem, but carbapenem resistance was found by oprD mutation-driven and the AmpC overproducing isolates in Tehran, Iran
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Indice:
Méditerranée orientale
Sujet Principal:
Pseudomonas aeruginosa
/
Brûlures
/
Multirésistance aux médicaments
/
Réaction de polymérisation en chaine en temps réel
/
Mutation
Limites du sujet:
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
J. Epidemiol. Glob. Health
Année:
2017
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