Phylogenetic identification ofburkholderia cepacia isolates from human chest infection from different hospitals

ABSTRACT

Phylogenetic analysis of Burkholderia cepacia was achieved based on the variation of p-Lactamases production and antibacterial reactivity to different antibiotics. Sub inhibitory concentrations of augmentin down-regulates the production and/or release of exoprotein and this effect increase with increasing of the tested concentration. ESBLs was detected using double disc synergy test and combined ESBL test. Effect of reactivity of B. cepacia spp was influenced by antibiotic where Amoxicillin/ clavulanic acid showed the highest inhibitory effect and in turn clear zone dimension. Also, TEM-1 and SHV-1 genes electrophoretic pattern detected by both PFGE and RAPD. Different isolates showed a similarity to standard B. cepacia ranging from 94-95 %. Data recorded revealed the p-Lactamases genes showed a variable protein bands pattern revealing reactivity to antibiotics