Cloning, expression and purification of pseudomonas putida ATCC12633 Creatinase

ABSTRACT

Background: Pseudomonas putida [P. putida] ATCC12633 can produce creatinase. It is a microbial enzyme which degrades creatinine in bacteria and provides source of carbon and nitrogen. Also, this enzyme is used in the enzymatic measurement of creatinine concentration for diagnosis of renal and muscles functions and diseases. Our purpose was recombinant production of creatinase for using in clinical measurement of serum or urine creatinine