IFN-gamma and IL-2 responses to recombinant AlaDH against ESAT-6/CFP-10 fusion antigens in the diagnosis of latent versus active tuberculosis infection

ABSTRACT

Background: Discriminating latent tuberculosis infection [LTBI] from active TBI may be challenging. The objective of this study was to produce the recombinant L-alanine dehydrogenase [AlaDH] antigen and evaluate individuals with LTBI, those with active TBI, and uninfected individuals by enzyme-linked immunospot assay [ELISPOT] in order to distinguish LTBI from active TBI