Real-time PCR: an appropriate approach to confirm ssDNA generation from PCR product in SELEX process

ABSTRACT

Background: Aptamers are single stranded DNA [ssDNA] or RNA molecules. The potential of aptamers for binding to the different targets has made them be widely used as the preferred diagnostic and therapeutic tools. DNA aptamers present several advantages over the RNA oligonucleotides due to their higher stability, easier selection, and production. Selection of DNA aptamers which is facilitated through a systematic evolution of ligand by exponential enrichment [SELEX] method is much dependent on the successful conversion of double stranded DNA [dsDNA] to ssDNA