Expression of CD[14], CD[16] on peripheral blood monocytes in patients with type II diabetes mellitus

ABSTRACT

The aim of the present study was to investigate the percentage of CD[14+] and CD[16+] monocytes in patients with type II diabetes. Peripheral blood samples were obtained from 10 patients with type 2 diabetes [group I], 10 non-diabetic patients with chronic periodontitis [group II] and 7 systemically and periodontally healthy donors who served as controls [group III]. Patients in group I and group II were selected with nearly similar plaque, gingival and periodontal scores. EDITA-treated venous blood was collected from both patients and control subjects. Flow cytometry and appropriate anti-human CD14, CD16 monoclonal antibodies were used for phenotyping analysis of peripheral blood monocytes. The results of the present study showed that both group I and group II showed a significantly increased expression of membrane bound CD[14] [mCD[14]] and membrane bound mCD[16] [mCD[16]] when compared to healthy controls. On comparing group I [patients with type 2 diabetes] and group II [non-diabetic patients with periodontitis] a significant difference in favor of group I was observed. The results of the present study implies that the increased expression of membrane bound CD[14] and CD[16] receptors on peripheral blood monocytes in patients with type 2 diabetes may play an important role in the pathogenesis of this disease as well as the increased inflammatory process observed in these patients. Also the increased expression of these receptors in patients with periodontitis may indicate a role of these receptors in inflammatory response and periodontal tissue destruction observed in these patients