[Apoptotic effect of Kyprolis on multiple myeloma KMM-1 cells through p73-mediated induction of G1 cell cycle arrest]

Background and Aim: Since proteasome is strongly considered to be involved in the development and progression of a wide variety of hematological malignancies in particular, multiple myeloma, blockage of this hemostasis system with different types of proteasome inhibitors seems to be a promising way of treatment for multiple myeloma. In this study, we investigated the effect of Kyprolis, a new irreversible proteasome inhibitor [PI], on the survival rate of multiple myeloma -derived KMM-1 cell line

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Material and Methods: To evaluate whether inhibition of proteasome using Kyprolis could exert cytotoxic effect in multiple myeloma, KMM-1 cells were cultured with different concentrations [25-150 nM] of the inhibitor for 24 and 48 hours. Then trypan blue exclusion assay, MTT assay, flocytometric cell cycle analysis were performed and we evaluated gene expression changes associated with apoptosis

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Results: The results of this study demonstrated that Kyprolis induced both cytotoxic and anti- proliferative effects on KMM-1 cells. This inhibitor is able to reduce the cell survival and metabolic activity in a dose- and also time-dependent manner [p Conclusions: The results of this study clearly indicated that Kyprolis had anti-tumor activity against KMM-1 cells